Effects of c9, t11-conjugated linoleic acid on adhesion of human gastric carcinoma cell line SGC-7901

摘要

AIM: To investigate the effect of c9, t11-conjugated linoleic acid (c9, t11-CLA) on the adhesion of human gastric carcinoma cell line (SGC-7901). METHODS: SGC-7901 cells were at first treated with different concentrations (25, 50, 100, 200 μmol/L) of c9, t11-CLA and 1 mL/L ethanol (as a negative control) for 24 h. Using adhesion assay and Western blot, we investigated the ability of SGC-7901 cells to adhere to intracellular matrix and examined the expression of E-cadherin (ECD), α-catenin, intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in these cells. RESULTS: The attachment rate to laminin of SGC-7901 cells treated with different concentrations of c9, t11-CLA (0, 25, 50, 100, and 200 μmol/L) was 100.0 +- 3.3, 95.7 +- 4.0, 89.2 +- 4.6, 87.9 +- 6.1, and 65.9 +- 5.8, respectively. The attachment rate to fibronectin was 100.0 +- 4.7, 96.8 +- 3.8, 94.5 +- 4.1, 76.5 +- 4.3, and 61.8+4.8, respectively. The attachment rate to Matrigel was 99.9 +- 6.6, 91.4 +- 6.8, 85.5 +- 7.4, 79.3 +- 5.6, and 69.6 +- 5.1, respectively. Besides, c9, t11-CLA could increase the level of ECD and α-catenin, and decrease the level of ICAM-1 and VCAM-1 in SGC-7901 cells. CONCLUSION: c9, t11-CLA can reduce the adhesion of human gastric carcinoma cells to laminin, fibronectin and Matrigel. c9, t11-CLA can increase the level of ECD and α-catenin, and decrease the level of ICAM-1 and VCAM-1 in human gastric carcinoma cells.