Role of transforming growth factor-β pathway in the mechanism of wound healing by saponin from Ginseng Radix rubra

摘要

The effects of saponin from Ginseng Radix rubra o extracellular matrix metabolism, the activation and synthesis of TGF-βl, and the modification of TGF- receptor in fibroblasts were examined to elucidate the contribution of the TGF-β pathway to the mechanism of wound heading by saponin. Fibronectin synthesis was analysed by the immunoprecipitation method. Activation and synthesis of TGF-β1 were measured by ELISA. The expression of TGF-β receptors in fibroblasts were examined at Protein and RNA levels by the cross-linking method and Northern blot analysis, respectively.