Preferential expression of the neuropeptide Y Y_1 over the Y_2 receptor subtype in cultured hippocampal neurones and cloning of the rat Y_2 receptor

摘要

Neuropeptide Y(NPY) and NPY receptors are most abundant in the hippocampal formation where they modulate cognitive functions. Expression of NPY receptors in rat cultured primary hippocampal cells was investigated in the present study by use of combined molecular, pharmacological and immunohistochemical approaches, including the cloning of the rat Y_2 receptor described here for the first time. More than 70/100 of the hipprocampal neurones were endowed with [`125I]-[Leu~31, Pro~34] PYY Y_1 -like Receptor silver grain accumulations and Y_1 receptor immunostaining. These radio- and immuno-labelling Signals were distributed over cell bodies and processed of bipolar. Stellate and pyramidal-like neuronal Cells, as confirmed by neurone-specific enolase and MAP-2 staining.