Enzymatic Synthesis of Fructooligosaccharides from Date By-products Using an Immobilized Crude Enzyme Preparation of β-D-fructofuranosidase from Aspergillus awamori NBRC 4033

摘要

Aqueous extracts from date by-products of the sucrose-rich variety "Deglet Nour" were used as a starting substrate to achieve the enzymatic synthesis of fructooligosaccharides (FOS) commonly used as prebiotics. A crude P-fructofuranosidase (Ffase) preparation from Aspergillus awamori NBRC4033 was immobilized on chitosan by covalent binding through glutaraldehyde linkages (Yi = 88%, Ya = 54%), and used for this purpose. The effect of water-extraction volume on the FOS synthesis by trans-fructosylation was studied. It was found that 150 mL/100 g of date by-products gave the best FOS concentration and productivity (123 g/L and 18.5 g/h/100g respectively), related to an optimal sucrose conversion of 53.26%. The main FOS product was purified via a biogel-P2 gel filtration column. Its structure was determined as 1-kestose: a-D-glucopyranosyl-(1→2)-β-D-fructofuranosyl-(2→1 )-β-D-fructofuranoside by combination of ~1H, ~(13)C and 2D-NMR techniques. Our results provide new insights into the enzymatic synthesis of FOS from an alternative source of sucrose, namely date by-products.