Lysine limitation alters the storage pattern of protein, lipid and glycogen in on-growing Atlantic salmon

摘要

This experiment aimed to test the interaction of lysine limitation with nutrient accretion and muscle carnitine depot in Atlantic salmon. Fish were fed adequate or low-lysine diets for 3 months. Lysine intake was significantly less (48%) in fish fed the low-lysine diet as compared with that fed the adequate one. There was no difference in dietary amino acids between treatments, with the exception of lysine. The lower lysine intake was reflected in plasma free lysine being 52% less while the free lysine concentration in the liver and muscle were unaffected.Although there was no significant difference between voluntary feed intakes among treatments, fish fed the low-lysine diet had reduced growth, protein and energy deposition as compared with fish fed the adequate lysine diet. White trunk muscle contained more glycogen and less protein in fish fed the low-lysine diet while no difference in lipid was observed. The livers from fish fed the low-lysine diet contained less glycogen and slightly more fat and protein than the livers from fish fed the adequate lysine diet. Lysine limitation reduced carnitine in the liver without affecting muscle carnitine depot. Thus, low-lysine diets did not likely affect the fatty acid oxidation capacity. This fact was supported by unaffected fatty acid profiles and lipid classes between treatments during the 3-month study. In conclusion, lysine limitation does not deplete the muscle carnitine depot during the on-growing seawater phase of Atlantic salmon, but affects the deposition pattern of nutrients.