首页> 外文期刊>Annals of Biomedical Engineering >Real-Time Imaging of Perivascular Transport of Nanoparticles During Convection-Enhanced Delivery in the Rat Cortex
【24h】

Real-Time Imaging of Perivascular Transport of Nanoparticles During Convection-Enhanced Delivery in the Rat Cortex

机译:大鼠皮层对流增强传递过程中纳米粒子的血管周围运输的实时成像。

获取原文
获取原文并翻译 | 示例
获取外文期刊封面目录资料

摘要

Convection-enhanced delivery (CED) is a promising technique for administering large therapeutics that do not readily cross the blood brain barrier to neural tissue. It is of vital importance to understand how large drug constructs move through neural tissue during CED to optimize construct and delivery parameters so that drugs are concentrated in the targeted tissue, with minimal leakage outside the targeted zone. Experiments have shown that liposomes, viral vectors, high molecular weight tracers, and nanoparticles infused into neural tissue localize in the perivascular spaces of blood vessels within the brain parenchyma. In this work, we used two-photon excited fluorescence microscopy to monitor the real-time distribution of nanoparticles infused in the cortex of live, anesthetized rats via CED. Fluorescent nanoparticles of 24 and 100 nm nominal diameters were infused into rat cortex through microfluidic probes. We found that perivascular spaces provide a high permeability path for rapid convective transport of large nanoparticles through tissue, and that the effects of perivascular spaces on transport are more significant for larger particles that undergo hindered transport through the extracellular matrix. This suggests that the vascular topology of the target tissue volume must be considered when delivering large therapeutic constructs via CED.
机译:对流增强传送(CED)是一种有前途的技术,可用于管理无法轻易穿越血脑屏障到达神经组织的大型疗法。了解在CED期间大型药物构建体如何在神经组织中移动以优化构建体和递送参数,以使药物集中在目标组织中,并使目标区域外的泄漏最少,这是至关重要的。实验表明,脂质体,病毒载体,高分子量示踪剂和注入神经组织的纳米颗粒位于脑实质内血管的血管周围空间。在这项工作中,我们使用了双光子激发荧光显微镜来监测通过CED注入活的麻醉大鼠皮层中的纳米颗粒的实时分布。通过微流探针将标称直径为24和100 nm的荧光纳米粒子注入大鼠皮层。我们发现血管周空间为大对流纳米粒子通过组织的快速对流运输提供了高渗透性路径,并且血管周空间对运输的影响对于较大颗粒的受阻通过细胞外基质的运输更为重要。这表明当通过CED输送大型治疗构造时,必须考虑靶组织体积的血管拓扑。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号