Mouse embryo co-culture with autologous cumulus cells and fetal development post-embryo transfer

摘要

This study was conducted to examine the potential for implantation and sustainable fetal development of mouse embryos cultured from the pronuclear to blastocyst stage. Pronuclear embryos from ICR mice (Harlan Sprague-Dawley) were cultured in Sydney IVF sequential media (Cook) to the blastocyst stage in medium only or co-cultured with autologous cumulus cells. We also experimented with co-culture in 100?μL drops. Drop co-culture produced blastocyst formation rates with a mean of 47.0%, which was significantly higher (P?