TEMPORARY TREATMENT MEDIUM, TREATMENT KIT, EMBRYO DEVELOPMENTAL ARREST INHIBITOR, METHOD FOR INHIBITING EMBRYO DEVELOPMENTAL ARREST, METHOD FOR PRODUCING DEVELOPMENTAL ENGINEERING PRODUCT, TRANSFER METHOD, THERAPEUTIC METHOD, AND DEVELOPMENTAL ENGINEERING PRODUCT

摘要

Provided is a temporary treatment medium for inhibiting embryo developmental arrest due to manipulation of an embryo or the like. A temporary treatment medium according to the present invention reduces damage to an in-vitro culture due to manipulation thereof, said in-vitro culture containing one of pluripotent stem cells, reproductive cells, a fertilized egg, and an embryo or any combination thereof. For this purpose, the temporary treatment medium contains a cytoskeleton regulator and/or an apoptosis inhibitor. The cytoskeleton regulator and/or the apoptosis inhibitor is preferably an Rho kinase inhibitor. Specifically, a Rock inhibitor can be used as the Rho kinase inhibitor. The Rock inhibitor is, for example, Y-27632. The temporary treatment medium is used for treating an in-vitro culture for a specific period before and/or after manipulation involving damage thereto.