Sensitive Chemiluminescence Immunoassay by Capillary Electrophoresis with Gold Nanoparticles

摘要

ABSTRACT: This technical note describes a new chemilumi-nnescence immunoassay hyphenated to capillary electrophoresisn(CE-based CL-IA) with gold nanoparticles (AuNPs) techniquenfor biological molecules determination. AuNPs were used as anprotein label reagent in the light of its excellent catalytic effectnto the CL reaction of luminol and hydrogen peroxide. AuNPsnconjugate with antibody (Ab) to form tagged antibody (Ab*),nand then Ab* link to antigen (Ag) to produce an Ab*-Agncomplex by a noncompetitive immunoreaction. The mixture ofnthe excess Ab* and the Ab*-Ag complex was baseline separatednand detected within 5 min under the optimized conditions.nThis new protocol was evaluated with human immunoglobulinnG (IgG) as the target molecule. The calibration curve of IgG was in the range of 0.008-5 μg/mL with a correlation coefficient ofn0.995. The detection limit (S/N= 3) of IgG was 1.14u000110-3nμg/mL (7.1 pmol/L, 0.39 amol). The proposed AuNPs enhanced CE-nbased CL-IA method was successfully applied for the quantification of IgG in human sera from patients. It proves that the presentnmethod could be developed into a new and sensitive biochemical analysis technique.