Chemical-Vapor-Deposition-Based Polymer Substrates for Spatially Resolved Analysis of Protein Binding by Imaging Ellipsometry

摘要

ABSTRACT: Biomolecular interactions between proteinsnand synthetic surfaces impact diverse biomedical fields.nSimple, quantitative, label-free technologies for the analysisnof protein adsorption and binding of biomolecules are thusnneeded.Here, we report the use of a novel type of substrate,npoly-p-xylylene coatings prepared by chemical vapor de-nposition (CVD) polymerization, for surface plasmon reso-nnance enhanced ellipsometry (SPREE) studies and assessnthe reactive coatings as spatially resolved biomolecular sen-nsing arrays. Prior to use in binding studies, reactive coatings were fully characterized by Fourier transform infrared spectroscopy,nelectrochemical impedance spectroscopy, and ellipsometry. As a result, the chemical structure, thickness, and homogeneousncoverage of the substrate surface were confirmed for a series of CVD-coated samples. Subsequent SPREE imaging and fluorescencenmicroscopy indicated that the synthetic substrates supported detectable binding of a cascade of biomolecules. Moreover, analysisnrevealed a useful thickness range for CVD films in the assessment of protein and/or antigen-antibody binding via SPREE imaging.nWith a variety of functionalized end groups available for biomolecule immobilization and ease of patterning, CVD thin films arenuseful substrates for spatially resolved, quantitative binding arrays.