Clonal Variation In Pinot Noir Revealed By S-sap Involving Universal Retrotransposon-based Sequences

摘要

A modified S-SAP (sequence-specific amplified polymorphism) method with universal primers for retrotransposons was used to study Vitis vinifera cv. Pinot noir. Five Pinot noir clones (20Gm, 1-44Gm, 18Gm, 20-13Gm, l-84Gm) were analyzed by 30 S-SAP primer combinations employing five Msel- and six universal retrotransposon primers. Altogether 670 markers were generated, revealing 4.8% clonal variation and four out of five Pinot noir clones could be differentiated. This S-SAP method provides an efficient tool to randomly screen for polymorphisms produced through retrotransposition processes in the Vitis genome.