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首页> 外文期刊>American journal of enology & viticulture >An Upgraded Core Set of 11 SSR Markers for Grapevine Cultivar Identification: The Case of Berry-Color Mutants
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An Upgraded Core Set of 11 SSR Markers for Grapevine Cultivar Identification: The Case of Berry-Color Mutants

机译:用于葡萄品种鉴定的11个SSR标记的升级核心集:以浆果色突变体为例

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摘要

This research focused on the development of a molecular assay, based on the amplification of 11 simple sequence repeats, that is useful to combine varietal identification with skin color discrimination of less-colored/colorless mutants from a black wild-type cultivar (like Pinots). The proposed molecular toolkit was reliable using both purified and unpurified DNA obtained from leaves, thereby speeding up the time required for laboratory analyses and lowering the costs of the molecular outcome.
机译:这项研究的重点是基于11个简单序列重复序列的扩增的分子检测技术的发展,该技术可用于将品种鉴定与来自黑色野生型品种(如黑皮诺)的较少颜色/无色突变体的肤色识别相结合。拟议的分子工具包使用从叶片获得的纯化和未纯化的DNA都是可靠的,从而加快了实验室分析所需的时间,并降低了分子结果的成本。

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