Demethylation capacity of human fetal adrenal mitochrondrial cytochrome P-450 in vitro

摘要

AIM: To explore the capacity and characteristics of adrenal mitochondria to metabolize xenobiotics in vitro in human fetus. METHODS: Subcellular fractions of fetal adrenal were prepared by differential centrifuga- tion. Mitochondrial P-450 system was proved by spectral analyses and SDS-PAGE. The formaldehyde formation contents were measured with Nash reagent. CONCLUSION: Fetal adrenal mitochondria, with Multiple P-450 isoforms and greater capacity of Demethylation, play a role in drug-metabolism during Fetal development.