Cloning, purification, crystallization and preliminary crystallographic analysis of the tandem tudor domain of Sgf29 from Saccharomyces cerevisiae

摘要

The protein Sgf29 has been identified as a subunit of the SAGA (Spt–Ada–Gcn5 acetyltransferase) histone acetyltransferase complex in Saccharomyces cerevisiae, which is conserved from yeast to humans. The tandem tudor domain at the C-terminus of Sgf29 was crystallized using the hanging-drop vapour-diffusion method and the crystals diffracted to 1.92 Å resolution. The crystals belonged to space group P2₁2₁2₁, with unit-cell parameters a = 49.76, b = 95.10, c = 114.43 Å, and are estimated to contain one protein molecule per asymmetric unit.