Process for the production of the enzyme antileucu00eamica asparaginase from the cloning of the gene AsP3 of Saccharomyces cerevisiae in a yeast metilotru00f3fica

摘要

Process for production of the enzyme antileucu00eamica asparaginase from the cloning of the gene AsP3 of Saccharomyces cerevisiae in a yeast metilotru00f3fica ".The present invention relates to a process for the production of the enzyme asparaginase II encoded by the gene AsP3 s.Cereviasiae, where the cloning and expression of the gene coding for the AsP3 asparaginase II of S.Cerevisiae is carried out in a yeast metilotru00f3fica, so as to obtain a yeast strain capable of producing high levels of asparaginase for use as an agent antileucu00eamico,Minimizing the side effects of asparaginases of bacterial origin and decreasing the cost of production of the enzyme.