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Sequence analysis and protein interactions of Arabidopsis CIA2 and CIL proteins

机译:拟南芥CIA2和CIL蛋白的序列分析和蛋白相互作用

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摘要

Cellular localizations of CIA2 and CIL fragments. Plasmids encoding various protein fragments, as labeled at left, were transformed into onion epidermal cells by means of particle bombardment. Samples were stained simultaneously with X-gluc and the nucleus-specific dye SYTOX. Schematic diagram of the constructs used in this experiment. CIA2-related constructs. CIL-related constructs. Left row, visualization of X-gluc staining; middle row, visualization of SYTOX staining; right row, superimposition of X-gluc and SYTOX staining. Scale bars in and represent 50 μm
机译:CIA2和CIL片段的细胞定位。如左图所示,通过粒子轰击将编码各种蛋白质片段的质粒转化为洋葱表皮细胞。样品同时用X-gluc和核特异性染料SYTOX染色。本实验中使用的构建体的示意图。 CIA2相关的构建体。与CIL相关的构造。左行,X-葡萄糖染色的可视化;中排,SYTOX染色的可视化;右行,X-gluc和SYTOX染色的叠加。比例尺为50μm

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