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Molecular characterization of polish blueberry red ringspot virus isolate

机译:波兰蓝莓红环斑病毒分离株的分子表征

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摘要

In this study, we determined the complete sequence of the genomic DNA of a Polish isolate of Blueberry red ringspot virus (BRRSV24) and compared it with a Czech (Darrow 5), and the US isolates of the virus and those of other Caulimoviridae family. The genomic DNA of BRRSV24 consists of 8,265 nucleotides and encodes eight open reading frames (ORFs). The sequence homologies of the eight ORFs of BRRSV24 were from 95 to 98% in respect of Darrow 5 and from 91 to 98% in respect of the US isolates at the amino acid level. This high level of amino acid sequence identity within the coding regions among the Czech, the US and Polish BRRSV isolates is suggestive of their common origin.Electronic supplementary materialThe online version of this article (doi:10.1007/s11262-011-0679-4) contains supplementary material, which is available to authorized users.
机译:在这项研究中,我们确定了波兰蓝莓红环斑病毒(BRRSV24)分离株的基因组DNA的完整序列,并将其与捷克(Darrow 5)和该病毒的美国分离株以及其他Caulimoviridae家族的分离株进行了比较。 BRRSV24的基因组DNA由8,265个核苷酸组成,编码8个开放阅读框(ORF)。就氨基酸水平而言,BRRSV24的八个ORF的序列同源性相对于Darrow 5而言为95%至98%,相对于US分离物而言为91%至98%。在捷克,美国和波兰的BRRSV分离株之间的编码区内,这种高水平的氨基酸序列同一性暗示了它们的共同起源。电子补充材料本文的在线版本(doi:10.1007 / s11262-011-0679-4)包含补充材料,授权用户可以使用。

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