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Populations of doubled haploids for genetic mapping in hexaploid winter triticale

机译:六倍体冬季小黑麦的双倍单倍体种群用于遗传作图

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摘要

To create a framework for genetic dissection of hexaploid triticale, six populations of doubled haploid (DH) lines were developed from pairwise hybrids of high-yielding winter triticale cultivars. The six populations comprise between 97 and 231 genotyped DH lines each, totaling 957 DH lines. A consensus genetic map spans 4593.9 cM is composed of 1576 unique DArT markers. The maps reveal several structural rearrangements in triticale genomes. In preliminary tests of the populations and maps, markers specific to wheat segments of the engineered rye chromosome 1R (RM1B) were identified. Example QTL mapping of days to heading in cv. Krakowiak revealed loci on chromosomes 2BL and 2R responsible for extended vernalization requirement, and candidate genes were identified. The material is available to all parties interested in triticale genetics.Electronic supplementary materialThe online version of this article (10.1007/s11032-018-0804-3) contains supplementary material, which is available to authorized users.
机译:为了创建六倍体小黑麦的遗传解剖框架,从高产量的冬季小黑麦品种的成对杂交中发展了六个种群的双单倍体(DH)品系。这六个种群分别包含97至231个基因型DH系,共957个DH系。共有4593.9 cM的遗传图谱由1576个独特的DArT标记组成。这些图揭示了黑小麦基因组中的几个结构重排。在种群和地图​​的初步测试中,鉴定了工程黑麦1R(RM1B)小麦片段的特异性标记。 QTL到cv中标题的示例QTL映射。 Krakowiak揭示了2BL和2R染色体上的基因位点,这些基因位点负责扩展春化要求,并鉴定了候选基因。该材料可供所有对黑小麦遗传学感兴趣的各方使用。电子补充材料本文的在线版本(10.1007 / s11032-018-0804-3)包含补充材料,授权用户可以使用。

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