The extraction behavior of thymus and the composition of fractions prepared from this organ has been studied. Sequential extraction methods using 0.15 M NaCl followed by water gave information with respect to the weight fraction of cytoplasmic and nuclear constituents. Lipide, nucleic acid, and electrophoretic analysis of the extracts provided additional information. A less complex electrophoretic pattern was obtained from subsequent extracts in the sequence. Sucrose and saline dispersates obtained from tissue fragmented with either the Potter-Elvehjem homogenizer or in a Waring blendor were fractionated, using standard differential sedimentation methods. The fractions obtained by means of four different dispersion procedures were compared in terms of yield, chemical analysis, and electrophoretic composition. The quantity of material in thymus having the sedimentation characteristics of liver mitochondrial and microsomal fractions was remarkably small. Both the suspension medium employed and the method used to bring about a disruption of the cells in the tissue affected the yield of "particulate" material. The components present in the later extracts in the sequence, E4 to E7, in the case of sequential extraction study resembled with respect to chemical composition and electrophoretic characteristics, the microsome fraction prepared by differential sedimentation methods. About 76 per cent of the PNA in the tissue appeared to be in the cytoplasm. The remaining 24 per cent PNA was found in the nucleus and accounted for 1.7 per cent of nucleus on a dry weight basis. From 75 to 88 per cent of cytoplasmic PNA was extracted from the tissue and 76 to 94 per cent of the PNA in the extract was found in the final supernatant solutions, depending upon the dispersion methods and suspension medium used in the extraction procedure. The composition of the final supernatant fractions using differential sedimentation methods were comparable in terms of electrophoretic properties, protein concentration, nucleic acid content, and fractionation behavior to saline extracts E1 to E3, of thymus used in earlier studies.
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机译：研究了胸腺的提取行为和由该器官制备的馏分的组成。先后使用0.15 M NaCl和水的顺序萃取方法得出有关细胞质和核成分重量分数的信息。提取物的脂质，核酸和电泳分析提供了更多信息。从序列中的后续提取物中获得了不太复杂的电泳图谱。使用标准微分沉降法，分馏从用Potter-Elvehjem匀浆器或Waring混合器破碎的组织中获得的蔗糖和盐水分散液。比较了通过四种不同分散程序获得的馏分的产率，化学分析和电泳组成。胸腺中具有肝线粒体和微粒体部分沉降特性的物质数量非常少。使用的悬浮介质和用于破坏组织中细胞的方法都影响“颗粒”材料的产量。在顺序提取研究的情况下，序列E4至E7的后续提取物中存在的成分在化学组成和电泳特性方面类似于通过微分沉淀法制备的微粒体级分。组织中约有76％的PNA出现在细胞质中。剩余的24％PNA存在于细胞核中，占干重的1.7％。从组织中提取了75％至88％的细胞质PNA，并且在最终的上清液中发现提取物中的76％至94％的PNA，取决于提取程序中使用的分散方法和悬浮介质。使用差异沉淀法的最终上清液馏分的组成与早期研究中使用的胸腺的盐提取物E1至E3相比，在电泳特性，蛋白质浓度，核酸含量和分离行为方面具有可比性。
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