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A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method

机译：重叠延伸PCR的嵌套拼接提高了此标准方法的特异性

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摘要

BackgroundSplicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function.

机译：背景技术通过重叠延伸拼接（SOE）PCR进行的拼接用于在酶的编码序列中产生突变，以研究特定残基在蛋白质结构和功能中的作用。

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期刊名称 Iranian Journal of Biotechnology
作者
Ali Asghar Karkhane; Bagher Yakhchali; Ferdous Rastgar Jazii; Bijan Bambai; Saeed Aminzadeh; Fatemeh Rahimi;
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作者单位

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年(卷),期 2015(13),2
年度 2015
页码 56–59
总页数 4
原文格式 PDF
正文语种
中图分类生物学 ;
关键词
Nested-Splicing by Overlap Extension PCR polymerase Chain Reaction Site-directed mutagenesis;

机译：通过重叠延伸PCR嵌套拼接;聚合酶链反应;定点诱变;

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专利

1. A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method [J] . Aminzadeh Saeed, Bambai Bijan, Karkhane Ali Asghar, Iranian Journal of Biotechnology . 2015 ,第2期

机译：通过重叠延伸PCR的嵌套拼接提高了此标准方法的特异性
2. Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis [J] . Hasnain Hussain, Nikson Fatt-Ming Chong BioMed research international . 2016 ,第23期

机译：改进位点定向诱变的组合重叠扩展PCR方法
3. An Improved Method of Gene Synthesis Based on DNA Works Software and Overlap Extension PCR [J] . Bingxue Dong, Runqian Mao, Baojian Li, Molecular Biotechnology . 2007 ,第3期

机译：基于DNA Works软件和重叠延伸PCR的基因合成改进方法
4. End-Specific PCR (ES-PCR) and Helper-Dependent Chain Reaction (HDCR) for Sensitive and Specific Detection of Cancer-Derived DNA [C] . Jason Ross, Susan Mitchell, Konsta Duesing, International Molecular Medicine Tri-Conference. . 2016

机译：特异性PCR（ES-PCR）和辅助依赖链反应（HDCR），用于敏感的癌症衍生的DNA检测
5. Detection of Escherichia coli ATCCRTM 8739(TM) and Aspergillus brasiliensis ATCCRTM16404(TM) in Raw Materials and Pharmaceutical Products Using the Real-Time PCR in Comparison with Standard Conventional Microbiological Methods. [D] . Garcia, Elsie Jacqueline Hernandez. 2016

机译：与标准常规微生物学方法相比，使用实时PCR检测原料药和药品中的大肠杆菌ATCCRTM 8739™和巴西曲霉ATCCRTM16404™。
6. Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis [O] . Hasnain Hussain, Nikson Fatt-Ming Chong 2006

机译：结合的重叠延伸PCR方法改进定点定向诱变
7. A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method [O] . Ali Asghar Karkhane, Bagher Yakhchali, Ferdous Rastgar Jazii, 2015

机译：通过重叠扩展PCR嵌套拼接改善了该标准方法的特异性

A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method

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