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METHODS OF DIAGNOSING DISEASE USING OVERLAP EXTENSION PCR
METHODS OF DIAGNOSING DISEASE USING OVERLAP EXTENSION PCR
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机译:重叠延伸PCR诊断疾病的方法
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摘要
A method of diagnosing a disease in a subject in need thereof is disclosed. The method comprises:(a) linking a plurality of non-contiguous DNA fragments from a sample of the subject to generate a polynucleotide product, the linking being effected by contacting the plurality of non-contiguous DNA fragments with a multiplex overlap-extension primer mix under conditions that allow simultaneous linkage of the DNA fragments and amplification of the polynucleotide product, wherein the primer mix comprises two flanking primers and at least one linker primer; and(b) determining a sequence of the polynucleotide product, wherein the sequence is indicative of the disease.展开▼