End-Specific PCR (ES-PCR) and Helper-Dependent Chain Reaction (HDCR) for Sensitive and Specific Detection of Cancer-Derived DNA

摘要

We have developed End-Specific PCR (ES-PCR) and Helper-Dependent Chain Reaction (HDCR), two PCR-based techniques particularly useful for the detection of minute quantities of target DNA within a vast excess of non-target DNA, such as in circulating tumor DNA and biopsy sample applications. These technologies have been applied, in particular, to the development of bisulfite-free DNA methylation assays to detect cancer-derived DNA. Recently, this process has been further developed into a deep sequencing platform which integrates DNA methylation biomarker discovery and clinical assay development, accelerating the path from discovery to clinical utility.