首页> 美国卫生研究院文献>Journal of Bacteriology >Immunogenicity of Irradiated Salmonella typhimurium Cells and Endotoxin
【2h】

Immunogenicity of Irradiated Salmonella typhimurium Cells and Endotoxin

机译:辐照的鼠伤寒沙门氏菌细胞和内毒素的免疫原性

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-, or alcohol-killed preparations. An amount of 5 Mrad is about a 50% end point in terms of inactivation of the lethal lipopolysaccharide or cell-associated determinants. The fever response to radiation-killed salmonellae decreased between 1- and 20-Mrad exposure. The immunogenicity of 1-Mrad-treated cells usually exceeded that of nonirradiated preparations in mouse-protection tests. With increasing radiation doses, there was a dramatic decrease in, but not an abolition of, immunogenicity. Preparations exposed to 20 Mrad which were nonlethal afforded significant protection. The results are interpreted as a reflection of a dissociation of the primary and secondary toxic determinants of endotoxin after irradiation. The data indicate the potential value of radiation sterilization as a means of production of Salmonella vaccine.
机译:研究了高剂量辐射(1、5或20 Mrad)对鼠伤寒沙门氏菌细胞和内毒素的毒性,热原性和免疫原性的影响。暴露于1、5或20 Mrad后,毒性逐渐降低。暴露于1-Mrad的细胞的致死作用大于经热,丙酮或酒精杀死的制剂的致死作用。就致死性脂多糖或细胞相关决定簇的失活而言,5 Mrad的量约为端点的50%。辐射杀死的沙门氏菌的发烧反应在1到20-Mrad暴露之间降低。在小鼠保护试验中,经1-Mrad处理的细胞的免疫原性通常超过未辐照制剂的免疫原性。随着放射剂量的增加,免疫原性显着降低,但并没有消除。暴露于20 Mrad的非致命性制剂具有明显的保护作用。结果被解释为辐射后内毒素的主要和次要毒性决定因素解离的反映。数据表明放射灭菌作为沙门氏菌疫苗生产手段的潜在价值。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号