Plant-derived protein hydrolysates have potential applications in nutrition. Rice protein hydrolysates (RPHs), an excellent source of proteins, have attracted attention for the development of cosmeceuticals. However, few studies have reported the potential application of RPH in analysis, and this study examined their antioxidant activities and the inhibitory activities of skin aging enzymes. The results indicated that the total phenolic and flavonoid concentrations were 2.06 ± 0.13 mg gallic acid equivalent/g RPHs and 25.96 ± 0.52 µg quercetin equivalent/g RPHs, respectively. RPHs demonstrated dose-dependent activity for scavenging free radicals from 1,1-diphenyl-2-picrylhydrazyl [half-maximal inhibitory concentration (IC50) = 42.58 ± 2.1 mg/g RPHs] and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (IC50 = 2.11 ± 0.88 mg/g RPHs), dose-dependent reduction capacity (6.95 ± 1.40 mg vitamin C equivalent/g RPHs) and oxygen radical absorbance capacity (473 µmol Trolox equivalent/g RPHs). The concentrations of the RPH solution required to achieve 50% inhibition of hyaluronidase and tyrosinase activities were determined to be 8.91 and 107.6 mg/mL, respectively. This study demonstrated that RPHs have antioxidant, antihyaluronidase, and antityrosinase activities for future cosmetic applications.
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机译:植物衍生的蛋白质水解产物具有营养的潜在应用。水稻蛋白质水解酸盐(RPH),蛋白质优异的来源,引起了化妆品的发展的关注。然而,很少有研究报告潜在的RPH在分析中应用,并且该研究检查了它们的抗氧化活性和皮肤衰老酶的抑制作用。结果表明,总酚类和黄酮浓度均为2.06±0.13mg Gallic酸当量/ g rphs,分别为25.96±0.52μg槲皮素等效/ g rphs。 RPHS证明了从1,1-二苯基-2-富铬酰基[半最大抑制浓度(IC50)= 42.58±2.1mg / g rphs]和2,2'-azino-bis(3-乙基苯并噻唑啉-6-磺酸)(IC50 = 2.11±0.88 mg / g rphs),剂量依赖性降低能力(6.95±1.40mg维生素C当量/ g rphs)和氧自由基吸收能力(473μmoltrolox当量/ g rphs) 。确定透明质酸酶和酪氨酸酶活性的50%抑制所需的RPH溶液的浓度分别为8.91和107.6mg / ml。该研究表明,用于未来化妆品应用的RPHs具有抗氧化剂,抗酸核苷酸酶和亚氨苯胺酶活性。
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