LINC-PINT impedes DNA repair and enhances radiotherapeutic response by targeting DNA-PKcs in nasopharyngeal cancer

摘要

a The fold change of LINC-PINT expression in NPC cells (HNE1, HONE1, CNE1, and CNE2) compared with that in immortalized nasopharyngeal epithelial cell line NP69 by qRT-PCR analysis. b The expression of LINC-PINT in NPC tumor tissues from our data (left) and the GDS3341 dataset (right). c In our data, the area under the ROC curve showing the discriminatory power of the LINC-PINT expression to predict the risk of NPC. d Correlation between LINC-PINT expression and treatment efficacy. CR complete response, PR partial response, SD stable disease, PD progressive disease. e Correlation between LINC-PINT expression and expressions of oncogenes (KRAS, MMP1, MYC, and TP53) in the NPC samples from the GDS3341 dataset. f HNE1 and HONE1 cells overexpressed LINC-PINT and verified by qRT-PCR. g LINC-PINT overexpression inhibited colony formation in NPC cells. Representative images (left) and quantitative analyses (right) are shown. h LINC-PINT overexpression suppressed cell proliferation in NPC cell lines, which are detected by CCK-8 assay. i Effect of LINC-PINT overexpression on the cell cycle progression. j Hoechst staining (green fluorescence) was also used to detect the effect of LINC-PINT on changes in apoptosis (up), Scale bar = 100 μm. Statistical diagrams show significant differences (bottom). Data represent the mean ± SEM from three independent experiments (a–j). Significance calculated with Student’s T test. *P < 0.05, **P < 0.01, ***P < 0.001. Data shown are mean ± SEM.