Supplier-origin mouse microbiomes significantly influence locomotor and anxiety-related behavior body morphology and metabolism

摘要

a Dot plot showing the difference in microbial fecal richness (p < 0.001, F = 10.1), as represented by the Chao-1 index, in 16-week-old, female (F) and male (M) CD-1 mice colonized with fecal microbiome 1 (FM1), FM2, FM3, or FM4 (n = 5–6/sex/FM), and used in phenotyping tests. b Dot plot showing the difference in microbial fecal richness (p < 0.001, F = 48.5), as represented by the Chao-1 index, in 6 to 12-week-old, female (F) and male (M) CD-1 mice colonized with FM1, FM2, FM3, or FM4 (n = 12/sex/FM), collected from the parent colonies prior to shipping. c Stacked bar chart showing taxonomic diversity, at the level of genus, in female and male CD-1 mice (n = 5-6/sex/FM) harboring one of four supplier-origin gut microbiomes (FM1 through FM4). d Three-dimensional principal coordinate analysis (PCoA) plot, based on Jaccard similarities, showing β-diversity captured by the first three coordinates, of phenotyped mice (darker symbols, n = 5–6/sex/FM) and parent colonies (lighter symbols, n = 12/sex/FM). e Venn diagram showing the number of ASVs detected in at least one mouse of the various FM groups. p and F values represent the main effects of FM in two-way ANOVA, and post hoc comparisons via Holm–Sidak method; significant pairwise FM-dependent differences are denoted by different letters, underlying bar charts represent mean ± SE.