miRNA-126-3p carried by human umbilical cord mesenchymal stem cell enhances endothelial function through exosome-mediated mechanisms in vitro and attenuates vein graft neointimal formation in vivo

摘要

Characterization of hucMSCs and HUVECs. a hucMSCs formed cell colony 3 days following isolation. b hucMSCs exhibited a fibroblast-like shape at passage 3. c hucMSCs were stained with Oil Red O to assess their adipogenic ability. d hucMSCs were stained with Alizarin Red to assess their osteogenic ability. e Flow cytometry analysis suggested that cultivated cells expressed strongly expressed CD29, CD44, CD90, CD105, and CD166 and HLA-DR but did not express CD34 and CD45. f Immunostaining of hucMSCs showing CD31 (red) and vWF (green) g staining. h Cell nuclei were stained with DAPI. i The merged images