Method for the Simultaneous Primary-Isolation and Expansion of Endothelial Stem/Progenitor Cell and Mesenchymal Stem Cell Derived From Mammal Including Human Umbilical Cord

摘要

Disclosed herein is a method for isolating and culturing the endothelial stem/progenitor cells and mesenchymal stem cells derived from the umbilical cord of mammals, including human beings. More specifically, disclosed are a method for isolating and culturing endothelial stem/progenitor cells and mesenchymal stem cells at high purity from the umbilical cord of mammals, including human beings, and preferably from the human umbilical cord, as well as endothelial stem/progenitor cells and mesenchymal stem cells, isolated and cultured according to said method, and a method for freezing and thawing the isolated and cultured cells. According to the disclosed method, endothelial stem/progenitor cells and mesenchymal stem cells can be easily isolated and purified with high purity from umbilical cord, and can be cultured with high viability for a long period of time.