Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts

摘要

CAF EVs promoted SACC lung metastasis and pre-metastatic niche formation. a FAP expression and quantification in the stroma of human SACC with or without lung metastasis. Scale bar = 50 μm. b Metastatic SACC-LM cells were detected by immunofluorescent staining with anti-human Pan CK antibody. C57BL/6 J mice were pre-treated with SACC-LM EVs (n = 5), CAF-A1 EVs (n = 4) and CAF-A2 EVs (n = 5). Scale bar = 50 μm. c Immunofluorescence staining and quantification of FN, LOX and MMP9 in lung sections from tumor-free mice injected every other day for 3 days with the indicated EVs (n = 5 per group). Immunofluorescence staining and quantification of VEGFR1+ cells in lung sections from tumor-free mice injected every other day for 14 days with the indicated EVs (n = 5 per group). Scale bar = 50 μm. * P < 0.05, **