Application of empirical Peleg model to study the water adsorption and mineral desorption of cocoa (Theobroma cacao) beans in water and sodium bicarbonate during debittering process

摘要

Instrumentation The chromatography was carried out using a Shimadzu system composed of SCL 10A VP Controller, LC 10AD pumps, SIL 10A Auto sampler injector, Prontosil 120-3-C18 ACE-EPS column oven, SPD M10AVP photodiode array detection (DAD) with a UV–Vis Detector system (all from Shimadzu, Kyoto Japan). Theobromine was separated on a reversed C18 column (150 × 4.6 mm; 3 µm id.; particle size, 3 mm) from BISCHOFF Analysentechnik und – geräte GmbH Leonberg Germany. The chromatography was carried out using Methanol–water 45:55 (v/v) as mobile phase at a flow rate of 0.8 ml/min and the run time was 20 min. The column temperature was 30 °C and the analyte was detected at 220 nm.