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An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking

机译:描述由Pediococccus酸酐产生的抗微生物肽的有效数据集纯化和分子对接测定的作用方式

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摘要

Most of the probiotics Bacterial cells, express native antibacterial genes, resulting in the production of, antimicrobial peptides, which have various applications in biotechnology and drug development. But the identification of antibacterial peptide, structural characterization of antimicrobial peptide and prediction on mode of action. Regardless of the significance of protein manufacturing, three individual factors are required for the production method: gene expression, stabilization and specific peptide purification. Our protocol describes a straightforward technique of detecting and characterizing particular extracellular peptides and enhancing the antimicrobial peptide expression we optimized using low molecular weight peptides. This protocol can be used to improve peptide detection and expression. The following are the benefits of this method, (DOI – https://doi.org/10.1016/j.ijbiomac.2019.10.196 [1]).
机译:大多数益生菌细菌细胞,表达天然抗菌基因,导致生产的抗微生物肽,其在生物技术和药物发育中具有各种应用。但鉴定抗菌肽,抗菌肽的结构表征和对动作模式的预测。无论蛋白质制造的重要性如何,生产方法需要三种个体因素:基因表达,稳定性和特异性肽纯化。我们的协议描述了检测和表征特定细胞外肽的直接技术,并增强我们使用低分子量肽优化的抗微生物肽表达。该方案可用于改善肽检测和表达。以下是此方法的好处(doi - https://doi.org/10.1016/j.ijbiomac.2019.10.196 [1])。

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