Tissue-scale tensional homeostasis in skin regulates structure and physiological function

摘要

a Schematic representation of ex vivo culture methods maintaining or releasing skin tensional homoeostasis. b Histological analyses of ex vivo cultured skin maintaining (upper columns) or releasing (lower columns) tensional homoeostasis. Macroscopic images (left panels) are shown. Scale bars, 1 mm. H&E staining (centre panels) and higher magnification images in each box area are shown (left panels). Scale bars, 1 mm or 50 µm. c Schematic representation of the construction methods of HSEs. d Images of the THS model. e Macroscopic (left) and H&E staining (second panels from the left) images of each model. Scale bars, 1 mm or 50 µm. f H&E staining of human skin and the THS model after 1, 3, 5 and 7 days of culture at the air–liquid interface. Scale bars, 50 µm. g, h Immunohistochemical analyses of human skin (upper columns) and the THS model (lower columns) after 7 days of culture at the air–liquid interface. Scale bars, 50 µm.