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Comparative evaluation for the globin gene depletion methods for mRNA sequencing using the whole blood-derived total RNAs

机译：使用全血源总RNA的MRNA测序的球蛋白基因耗尽方法的比较评估

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摘要

The overall experimental design is shown. Total RNA was extracted from six samples collected in Paxgene Blood Tubes and treated with DNaseI. Technical replicates of 1μg of each sample underwent depletion with one of the four kits and sequenced using the poly-A+ selection protocols. NEBgr, NEBNext® Globin & rRNA Depletion Kit; RZr, Ribo-Zero Plus rRNA Depletion Kit; GZr, Globin-Zero Gold rRNA Removal Kit

机译：显示了整体实验设计。从血管血管收集的六个样品中提取总RNA，并用DNASEI处理。用四个试剂盒中的一种并使用多A +选择方案进行测序，每个样品的技术复制耗尽。 Nebgr，Nebnext®Lobalin＆rrna耗尽套件; RZR，Ribo-Zero Plus RRNA耗尽套件; GZR，Globin-Zero Gold RRNA拆卸套件

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期刊名称 BMC Genomics
作者
Jin Sung Jang; Brianna Berg; Eileen Holicky; Bruce Eckloff; Mark Mutawe; Minerva M. Carrasquillo; Nilüfer Ertekin-Taner; Julie M. Cuninngham;
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作者单位

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年(卷),期 2020(-1),-1
年度 2020
页码 -1
总页数 9
原文格式 PDF
正文语种
中图分类遗传学;应用微生物学;
关键词

机译：mRNA-SEQ;球蛋白基因耗尽;rRNA;全血;

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专利

1. Comparative evaluation for the globin gene depletion methods for mRNA sequencing using the whole blood-derived total RNAs [J] . Jin Sung Jang, Brianna Berg, Eileen Holicky, BMC Genomics . 2020,第1期

机译：使用全血源总RNA的MRNA测序的球蛋白基因耗尽方法的比较评估
2. A comparative analysis of RNA sequencing methods with ribosome RNA depletion for degraded and low-input total RNA from formalin-fixed and paraffin-embedded samples [J] . Xiaojing Lin, Lihong Qiu, Xue Song, BMC Genomics . 2019,第1期

机译：具有核糖体RNA耗尽的RNA测序方法对来自福尔马林固定和石蜡包埋的样品的降解和低输入总RNA的比较分析
3. RNA-Seq of human whole blood: Evaluation of globin RNA depletion on Ribo-Zero library method [J] . Suzanne Fei, Chris Harrington, Jessica Minnier, Journal of biomolecular techniques :JBT. . 2020,第Suppl期

机译：人类全血的RNA-SEQ：核蛋白RNA耗尽对核素零库方法的评价
4. Finding the Association of mRNA and miRNA Using Next Generation Sequencing Data of Kidney Renal Cell Carcinoma [C] . Shib Sankar Bhowmick, Luis Rato, Debotosh Bhattacharjee International conference on advanced computational and communication paradigms . 2018

机译：使用肾肾细胞癌的下一代测序数据找到mRNA和miRNA的关联
5. A data processing strategy for the computational identification of novel mRNA transcripts and novel mRNA splice-forms from RNA-seq data generated by massively parallel sequencing. [D] . Darko, Samuel W. 2010

机译：一种从大规模并行测序产生的RNA-seq数据中计算鉴定新型mRNA转录物和新型mRNA剪接形式的数据处理策略。
6. A comparative analysis of RNA sequencing methods with ribosome RNA depletion for degraded and low-input total RNA from formalin-fixed and paraffin-embedded samples [O] . Xiaojing Lin, Lihong Qiu, Xue Song, 2019

机译：用福尔马林固定和石蜡包埋样品降解和低输入总RNA的核糖体RNA消耗RNA测序方法的比较分析
7. Next Generation Sequencing Analysis of Human Platelet PolyA plus mRNAs and rRNA-Depleted Total RNA [O] . Kissopoulou, Antheia, Jonasson, Jon, Lindahl, Tomas, 2013

机译：人血小板PolyA plus mRNA和rRNA耗尽的总RNA的下一代测序分析

Comparative evaluation for the globin gene depletion methods for mRNA sequencing using the whole blood-derived total RNAs

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