METHOD FOR EVALUATING GENE EXPRESSION-DEPRESSING EFFECT, AND METHOD FOR DETECTING CLEAVAGE OF mRNA

摘要

PPROBLEM TO BE SOLVED: To provide a method for directly detecting the presence or absence of the cleavage of mRNA or presence or absence of a bond by miRNA. PSOLUTION: This method for evaluating the gene expression-depressing effect is provided by comprising a process of performing a reverse transcription reaction by using a fluorescence-labeled primer and a cell introduced with siRNA or miRNA, or a cell-originated RNA containing an intrinsic miRNA as a template, a process of analyzing by detecting fluorescence generated from a first fluorescence-labeled substance used for the labeling of the fluorescence-labeled primer and measuring the containing ratio (molar ratio) or degree of elongation of an elongated product of the fluorescence-labeled primer in the reverse transcription reaction product, and based on the containing ratio or degree of elongation of obtained elongation product of the fluorescence-labeled primer, a process of evaluating the gene expression-depressing effect by the siRNA, or the like, wherein the hybridizing region with the fluorescence-labeled primer in the mRNA of a target gene is closer to the region of 3' terminal side than the expected cleavage site by the siRNA, or the like, or the expected bonding site by the miRNA. PCOPYRIGHT: (C)2011,JPO&INPIT