Critique of the pairwise method for estimating qPCR amplification efficiency: beware of correlated data!

摘要

qPCR fluorescence data displayed in Fig. ​Fig.2a2a in ref. [11]. The dilution factor is 2 for these curves, which represent the lowest 3 concentrations in the first C series. The horizontal dashed lines demarcate the exponential growth zone; this was set lower — 20-180 — for the full analysis in [11]. The RFU (relative fluorescence units) plateau values are ~ 600