Global targetome analysis reveals critical role of miR-29a in pancreatic stellate cell mediated regulation of PDAC tumor microenvironment

摘要

RNAseq analysis of miR-29a overexpressing hPSCs. a qPCR analysis for miR-29a expression in hPSCs transfected with miR-29a mimics (29a OE) as compared to hPSCs transfected with scramble control (CTRL). Numerical data are represented as average fold change (ΔΔCT) ± standard error of the mean (SEM); ***p < 0.001; n = 6. b Volcano plot of DEGs (log FC > 1 or < − 1, FDR < 0.05) in hPSC cells overexpressing miR-29a compared to controls. The horizontal axis represents log2 fold change between miR-29a overexpressing and control hPSCs. The negative log10 of the q-value is plotted on the vertical axis. Each point on the graph represents one gene. c A hierarchically clustered heatmap showing the expression patterns of the differentially expressed miR-29a direct target genes in the three replicates for each of miR-29a overexpressing (OE1, OE2 and OE3) and control (Control 1, Control 2, Control 3) mRNAs. Red and blue represent up- and downregulation respectively, and the color intensity represents the level of fold changes