A chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice

摘要

a BinJ/DENV2-prME chimeric virus genome schematic with the prM/E genes of DENV2 indicated in green and BinJV capsid (C) and NS genes in grey. b BinJ/DENV2-prME was purified via a potassium tartrate gradient, sedementing as a wide opalescent blue band. c SDS-PAGE (4–12%) analysis of gradient-purified BinJ/DENV2-prME. Flavivirus structural proteins (pre-membrane (prM), C, membrane (M) and envelope (E) protein) are indicated. Gels were derived from the same experiment and processed in parallel. d Negative-stain TEM image of BinJ/DENV2-prME at ×30,000 magnification, highlighting representative mature (open arrowheads) and immature (closed arrowheads) viral particles.