Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner

摘要

A, B Serum levels of KIAA1199 (A) and LMW-HA (MW < 100 kDa) (B) in normal subjects (n = 18), inactive RA (n = 27), and active RA (n = 33) patients were determined by ELISA. C Correlation between serum KIAA1199 and LMW-HA was obtained by the Pearson’s coefficient analysis (r = 0.4309, p < 0.0001, n = 78). D, E Synovial levels of KIAA1199 (D) and LMW-HA (E) in normal subjects (n = 9), inactive RA (n = 18), and active RA (n = 22) patients. F Linear correlation between KIAA1199 and LMW-HA in the synovial fluid (r = 0.6532, p < 0.0001, n = 49). G–I TNF-α (G), IL-1β (H), and IL-6 (I) levels in the synovial fluid of normal subjects (n = 9), inactive (n = 18), and active (n = 22) RA patients. J, K Correlation analysis of synovial TNF-ɑ (J), IL-1β (K), IL-6 (L), and KIAA1199, respectively (n = 49). All of the reactions were conducted in triplicate. Data were presented with mean ± SD.