RGD4C peptide mediates anti-p21Ras scFv entry into tumor cells and produces an inhibitory effect on the human colon cancer cell line SW480

摘要

Prokaryotic expression of recombinant proteins. (a) The sequences of the anti-p21Ras scFv, RGD4C-scFv, RGD4C-linker-scFv, and RGD4C-EGFP were separately ligated into the pET28a (+) vector to construct recombinant expression plasmids. (b) The insertion sequences were detected by polymerase chain reaction (PCR) analysis in E. coli BL21 (DE3). (*): unoptimized coding sequence. (c) SDS-PAGE analysis showed that the molecular weight of the anti-p21Ras scFv was 34 kDa, that of RGD4C-scFv was 35 kDa, that of RGD4C-linker-scFv was 36 kDa, and that of RGD4C-EGFP was 34 kDa. Codon optimization did not change the molecular weights of the expression products