Bioinformatics analysis of the gene expression profile of retinal pigmental epithelial cells based in single-cell RNA sequencing in myopic mice

摘要

Myopia is a very common eye condition that is rapidly increasing in occurrence. It is the most common eye disease in teenagers [1], and uncorrected ametropia is the main cause of moderate or severe visual impairment in people aged 50 years and above [2]. It is estimated that the number of patients with myopia will reach 4.758 billion by 2050, accounting for 49.8% of the global population [3]. The severity of a patient’s myopia is also associated with increased risk of visual impairment due to macular degeneration, cataracts, retinal detachment, and glaucoma caused by high myopia [4]. A decreased visual function in the early stage of myopia [5–7] has also been noted, but the molecular mechanism remains unclear. Due to the existence of a large number of genes that affect biological function, retinal pigmental epithelium (RPE) single-cell captured ribonucleic acid (RNA) sequencing technology was adopted to explore the molecular mechanism of RPE cell apoptosis in myopia and provide a theoretical basis for clinical guidance aimed at protecting early visual function in myopic patients.