Feeder-free method for obtaining retinal progenitor cells, retinal pigment epithelial cells and neural retinal cells

摘要

The present invention comprises the steps of (i) positioning the adherent culture of human pluripotent stem cells in a stem cell specific neuronal promoting media, wherein the culture lacks feeder cells; And (ii) maintaining said culture in said stem cell specific neuronal stimulating medium until pigmented cells and / or neuroepithelial-like structures appear. Human retinal progenitors It is about a method to obtain). Advantageously additional steps may be performed to obtain precursors of RPE cells and / or neural membranes.