Design and Evaluation of a Macroarray for Detection Identification and Typing of Viral Hemorrhagic Septicemia Virus (VHSV)

摘要

In the present report will be the first of a series of PCR-based macroarrays for the detection, typing, and quantification of several viruses of importance in fish aquaculture. The way of preparing the array—under patent process—differs from macroarrays reported by other researchers because it ensures its reliability after storage times up to 1 year at −25 °C. In this study, we show the results of the evaluation of a macroarray designed for the viral hemorrhagic septicemia virus (VHSV), of great importance for fish farmers worldwide, and the same procedure of validation is ongoing with macroarrays for other fish viruses.