A portable optical-fibre-based surface plasmon resonance biosensor for the detection of therapeutic antibodies in human serum

摘要

Effects of the three steps of the immobilization procedure as evaluated in real time in the ProteonXPR36 SPR instrument. For these studies, we used a bare gold chip pre-coated (out of the instrument) with lipoic acid. The figure shows the changes in the SPR signals (in resonance units) during the sequential 5 min flow with EDC/NHS (activation), followed by IgG or the anti-IFX antibody (immobilization) and ethanolamine (deactivation). The SPR signal measured at the end of the whole procedure indicates the immobilization level.