针对阵列样品的定量检测,构建了一种用于阵列样品检测的扫描式表面等离子体共振生物传感器.首先,基于平面棱镜耦合下的最佳旋转轴位置和双棱镜探测光路搭建了阵列扫描式表面等离子体共振生物传感器.然后,计算了可探测阵列样品的点密度.最后,以蒸馏水和浓度分别为5,10,15,20,25,30,35 mg/mL的葡萄糖溶液作为待测阵列样品进行了多样品点的表面等离子体共振实验.实验测得阵列样品的共振角分别为73.745,73.919,74.052,74.185,74.306,74.408,74.549,74.660°,显示葡萄糖溶液浓度与共振角线性关系良好,证明了该方法和装置的可行性.该装置对提高阵列样品点密度,实现高精度定量检测具有重要意义.%An scanning surface plasmon resonance biosensor was constructed to quantitatively detect array samples. First, based on the optimal rotation position in plane prism-coupling mode and a double-prism detection optical path, the scanning surface plasmon resonance biosensor for array sampleswas constructed. Then, the sample density of array samples was calculated. Finally, by taking the distilled water and glucose solution with different concentrations in 5, 10, 15, 20, 25, 30 and 35 mg/ mL as array samples to be measured, the multi-sample spots were detected by surface plasmon resonance method. Experimental results show that the resonance angles of array samples are 73. 745, 73. 919, 74. 052, 74. 185, 74. 306, 74. 408, 74. 549 and 74. 660? respectively, and the solution concentrations and their resonance angles show a good linear relation, which proves that the proposed method and device are feasibility. The device is significant for increasing the sample density of array samples and realizing the high precision detection.
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