pC6-2/caspase-6 system to purify glutathione-S-transferase-free recombinant fusion proteins expressed in Escherichia coli

摘要

( ) DNA sequences surrounding the MCS region of pGEX-4T-2 are depicted. ( ) Restriction digestion of pGEX-4T-2 with BamHI generates the sticky ends as depicted. ( ) Casp6F and Casp6R primers upon annealing generate the BamHI cohesive ends; therefore, they can be ligated directly into the BamHI digested vector. ( ) The DNA sequence of the pC6-2 vector at the MCS region upon ligation of the Casp6F and Casp6R annealed oligonucleotides into the pGEX-4T-2 is depicted. One of the BamHI sites is lost but that at the MCS region is regenerated in the pC6-2 vector.