Phylogeny of Anopheles (Kerteszia) (Diptera: Culicidae) Using Mitochondrial Genes

摘要

Identification of mosquito species is necessary for determining the entomological components of malaria transmission, but it can be difficult in morphologically similar species. DNA sequences are largely used as an additional tool for species recognition, including those that belong to species complexes. mosquitoes are vectors of human and simian malaria in the Neotropical Region, but there are few DNA sequences of species in public databases. In order to provide relevant information about diversity and improve knowledge in taxonomy of species in Peru, we sequenced part of the mitochondrial genome, including the cytochrome c oxidase I (COI) barcode region. Phylogenetic analyses structured all species of mosquitoes collected in Peru into a single clade, separate from the Brazilian species. The Peruvian clade was composed of two lineages, encompassing sequences from and . sequences were recorded for the first time in Peru, whereas sequences were for the first time published in the GenBank database. Sequences generated from specimens morphologically identified as clustered into three separate clades according to the collection localities of Serra do Mar, Serra da Mantiqueira, and Serra da Cantareira, confirming as a species complex, composed of at least three putative species.