Comparative Semen Microbiota Composition of a Stallion in a Taylorella equigenitalis Carrier and Non-Carrier State

摘要

Contagious equine metritis carriers have become a new cause of concern in horse stud farms. Their detection can result in significant financial loss and force owners to have their animals undergo antibiotic treatment. Current research has not been able to satisfactorily explain the appearance of carriers in agent-free farms. Studies made on microbial flora have given new insights into the diagnosis and treatment of different issues in animal systems. Next-generation sequencing (NGS) is a powerful tool that can draw an accurate picture of microbial flora. Therefore, the aim of this study was to compare the seminal bacterial composition of one stallion before and after being diagnosed with using NGS. Our results show that the microbial seminal flora visibly changed between the samples analyzed. Corynebacteriaceae, an opportunistic bacterial family, was more common in the infected sample. However, Porphyromonadaceae, a natural component in several tissues, was more abundant in the negative sample. Despite the constraints of a single-case study, these findings can open the door to new therapeutic tools, as flora transplants. Similarly, seminal flora analysis may foresee microbial shifts, letting practitioners take preventive actions before a potential outbreak. Furthermore, these actions would have the extra benefit of reducing the administration of antibiotics to treat an infection.