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Differential expression analysis comparing EBV uninfected to infected human cell lines identifies induced non-micro small non-coding RNAs

机译:比较未感染的EBV与感染的人类细胞系的差异表达分析可鉴定诱导的非微小非编码RNA

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摘要

Epstein–Barr virus (EBV) is a ubiquitous human herpes virus, which is implicated in cancer and various autoimmune diseases. This study profiles non-micro small non-coding RNA expression changes induced by latent EBV infection. Using small RNA-Seq, 346 non-micro small RNAs were identified as being significantly differentially expressed between EBV(+) BJAB-B1 and EBV(−) BJAB cell lines. Select small RNA expression changes were experimentally validated in the BJAB-B1 cell line as well as the EBV-infected Raji and Jijoye cell lines. This latter analysis recapitulated the previously identified induction of vault RNA1, while also finding novel evidence for the deregulation of several tRNAs and a snoRNA.
机译:爱泼斯坦-巴尔病毒(EBV)是一种普遍存在的人类疱疹病毒,与癌症和各种自身免疫性疾病有关。这项研究概述了由潜在的EBV感染诱导的非微细非编码RNA表达变化。使用小RNA-Seq,鉴定出346个非微小RNA在EBV(+)BJAB-B1和EBV(-)BJAB细胞系之间显着差异表达。选择的小RNA表达变化已在BJAB-B1细胞系以及EBV感染的Raji和Jijoye细胞系中进行了实验验证。后面的分析概括了先前确定的穹顶RNA1的诱导,同时还发现了一些tRNA和snoRNA失控的新证据。

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