The expression and localization of the V and W accessory proteins in porcine and human cells infected with Nipah Virus comparing the antiviral state and IFN signalling throughout a Nipah Virus infection.

摘要

Nipah Virus (NiV) is a zoonotic pathogen in the Paramyxoviridae family which infects several species with different virulence. The virus p gene codes for three proteins (P, V, and W) stated to have STAT1 binding activity, indicating potential of NiV to evade the IFN-STAT1 signalling pathway.In contrast to data published based on recombinant techniques, the intracellular localization and expression of NiV V/W by western blot and immunofluorescence detected the protein(s) and their co-localization with STAT1 only within the cytoplasm on infected porcine and human cells.The presented work shows that in vitro, NiV is sensitive to the antiviral action of IFN alpha, Comparison of human and porcine cells indicated that porcine cells established exogenous IFN-induced antiviral state at 18 hrs post treatment with 160 U/ml of IFN alpha while human cells needed 24 hrs and 329 U/ml.