Absence of parvalbumin increases mitochondria volume and branching of dendrites in inhibitory Pvalb neurons in vivo: a point of convergence of autism spectrum disorder (ASD) risk gene phenotypes

摘要

PV expression in 3–5 month old PV-EGFP mice. Sagittal section showing PV Pvalb neurons (red) in various brain regions of a PV-EGFP mouse. Sections are co-stained with DAPI (blue) to identify cell nuclei. The analyzed regions 1–8 obtained by microscopy are shown at higher magnification and were obtained by confocal microscopy in ( ); scale bar in ( ) 1000 μm. Confocal images of Pvalb neurons of a PV-EGFP mouse in the regions 1–8 stained for PV (upper row) and for EGFP (lower row), scale bar 50 μm. Strong correlation between green EGFP signals (in a.u.) and red PV signals (in a.u.) in the 8 investigated regions indicates that EGFP signals are a reliable proxy measure of PV concentrations. Note the larger SD values for the PV staining than for EGFP staining in low-PV expressing Pvalb neurons (DG, CA3, CA1). Representative standard curve using data from ( ) of (corrected) EGFP signals vs. PV concentrations previously reported in (from low to high) hippocampal Pvalb neurons, PC, and cerebellar basket cells. Estimation of PV concentrations in other Pvalb neuron subpopulations (for details, see suppl. material)